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Reconstitution in the apo-enzymes was carried out overnight at four °C prior to elimination of unbound metal by passage by way of a PD-10 column. The PD-10 column was pre-handled with dipicolinate to eliminate traces of unbound metallic after which washed with five column volumes of metallic cost-free HEPES buffer, pH 8.0. Immediately after elution, the focus of your steel-reconstituted enzyme was determined by UV absorbance as well as the metallic content material of the samples determined by ICP-MS.
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Naga acid is lethal, but can in fact be accustomed to neutralize other poisons. The fangs they drop on Demise can be used to brew poison antidotes and preventatives.
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Nagas are big flying hostile mobs located on coastal cliffs. They are venomous winged serpents that will break up acid at their enemies.
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